Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Identifieur interne : 000047 ( France/Analysis ); précédent : 000046; suivant : 000048Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
Auteurs : Victor M. Corman [Allemagne] ; Olfert Landt [Allemagne] ; Marco Kaiser [Allemagne] ; Richard Molenkamp [Pays-Bas] ; Adam Meijer [Pays-Bas] ; Daniel Kw Chu [République populaire de Chine] ; Tobias Bleicker [Allemagne] ; Sebastian Brünink [Allemagne] ; Julia Schneider [Allemagne] ; Marie Luisa Schmidt [Allemagne] ; Daphne Gjc Mulders [Pays-Bas] ; Bart L. Haagmans [Pays-Bas] ; Bas Van Der Veer [Pays-Bas] ; Sharon Van Den Brink [Pays-Bas] ; Lisa Wijsman [Pays-Bas] ; Gabriel Goderski [Pays-Bas] ; Jean-Louis Romette [France] ; Joanna Ellis [Royaume-Uni] ; Maria Zambon [Royaume-Uni] ; Malik Peiris [République populaire de Chine] ; Herman Goossens [Belgique] ; Chantal Reusken [Pays-Bas] ; Marion Pg Koopmans [Pays-Bas] ; Christian Drosten [Allemagne]Source :
- Eurosurveillance [ 1025-496X ] ; 2020.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), Flambées de maladies, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Techniques de laboratoire clinique ().
- MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus.
- isolement et purification : Coronavirus.
- virologie : Infections à coronavirus.
- Coronavirus, Flambées de maladies, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de laboratoire clinique.
English descriptors
- KwdEn :
- Clinical Laboratory Techniques (methods), Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Disease Outbreaks, Humans, RNA, Viral (analysis), Real-Time Polymerase Chain Reaction (methods), Sensitivity and Specificity.
- MESH :
- chemical , analysis : RNA, Viral.
- classification : Coronavirus.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Clinical Laboratory Techniques, Real-Time Polymerase Chain Reaction.
- virology : Coronavirus Infections.
- Disease Outbreaks, Humans, Sensitivity and Specificity.
Abstract
The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.
We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.
Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project.
The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
Url:
DOI: 10.2807/1560-7917.ES.2020.25.3.2000045
PubMed: 31992387
PubMed Central: 6988269
Affiliations:
- Allemagne, Belgique, France, Pays-Bas, Royaume-Uni, République populaire de Chine
- Angleterre, Berlin, Grand Londres, Hollande-Méridionale, Provence-Alpes-Côte d'Azur, Province d'Anvers, Région flamande
- Anvers, Berlin, Londres, Marseille, Rotterdam
- Université d'Aix-Marseille, Université d'Anvers
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PMC:6988269Le document en format XML
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id="aff7">Public Health England, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff5">University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name><affiliation wicri:level="4"><nlm:aff id="aff8">Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp</wicri:regionArea><orgName type="university">Université d'Anvers</orgName><placeName><settlement type="city">Anvers</settlement><region>Région flamande</region><region type="district" nuts="2">Province d'Anvers</region></placeName></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), 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Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31992387</idno><idno type="pmc">6988269</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269</idno><idno type="RBID">PMC:6988269</idno><idno type="doi">10.2807/1560-7917.ES.2020.25.3.2000045</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000F99</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000F99</idno><idno type="wicri:Area/Pmc/Curation">000F99</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000F99</idno><idno 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Corman</name><affiliation wicri:level="3"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Landt, Olfert" sort="Landt, Olfert" uniqKey="Landt O" first="Olfert" last="Landt">Olfert Landt</name><affiliation wicri:level="3"><nlm:aff id="aff2">Tib-Molbiol, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Tib-Molbiol, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kaiser, Marco" sort="Kaiser, Marco" uniqKey="Kaiser M" first="Marco" last="Kaiser">Marco Kaiser</name><affiliation wicri:level="3"><nlm:aff id="aff2">Tib-Molbiol, Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Tib-Molbiol, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Molenkamp, Richard" sort="Molenkamp, Richard" uniqKey="Molenkamp R" first="Richard" last="Molenkamp">Richard Molenkamp</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Meijer, Adam" sort="Meijer, Adam" uniqKey="Meijer A" first="Adam" last="Meijer">Adam Meijer</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Chu, Daniel Kw" sort="Chu, Daniel Kw" uniqKey="Chu D" first="Daniel Kw" last="Chu">Daniel Kw Chu</name><affiliation wicri:level="1"><nlm:aff id="aff5">University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Bleicker, Tobias" sort="Bleicker, Tobias" uniqKey="Bleicker T" first="Tobias" last="Bleicker">Tobias Bleicker</name><affiliation wicri:level="3"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Brunink, Sebastian" sort="Brunink, Sebastian" uniqKey="Brunink S" first="Sebastian" last="Brünink">Sebastian Brünink</name><affiliation wicri:level="3"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Schneider, Julia" sort="Schneider, Julia" uniqKey="Schneider J" first="Julia" last="Schneider">Julia Schneider</name><affiliation wicri:level="3"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Schmidt, Marie Luisa" sort="Schmidt, Marie Luisa" uniqKey="Schmidt M" first="Marie Luisa" last="Schmidt">Marie Luisa Schmidt</name><affiliation wicri:level="3"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Mulders, Daphne Gjc" sort="Mulders, Daphne Gjc" uniqKey="Mulders D" first="Daphne Gjc" last="Mulders">Daphne Gjc Mulders</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Van Der Veer, Bas" sort="Van Der Veer, Bas" uniqKey="Van Der Veer B" first="Bas" last="Van Der Veer">Bas Van Der Veer</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Van Den Brink, Sharon" sort="Van Den Brink, Sharon" uniqKey="Van Den Brink S" first="Sharon" last="Van Den Brink">Sharon Van Den Brink</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Wijsman, Lisa" sort="Wijsman, Lisa" uniqKey="Wijsman L" first="Lisa" last="Wijsman">Lisa Wijsman</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Goderski, Gabriel" sort="Goderski, Gabriel" uniqKey="Goderski G" first="Gabriel" last="Goderski">Gabriel Goderski</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Romette, Jean Louis" sort="Romette, Jean Louis" uniqKey="Romette J" first="Jean-Louis" last="Romette">Jean-Louis Romette</name><affiliation wicri:level="4"><nlm:aff id="aff6">Universite d Aix-Marseille, Marseille, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Universite d Aix-Marseille, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Ellis, Joanna" sort="Ellis, Joanna" uniqKey="Ellis J" first="Joanna" last="Ellis">Joanna Ellis</name><affiliation wicri:level="3"><nlm:aff id="aff7">Public Health England, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name><affiliation wicri:level="3"><nlm:aff id="aff7">Public Health England, London, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Public Health England, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff5">University of Hong Kong, Hong Kong, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong</wicri:regionArea><wicri:noRegion>Hong Kong</wicri:noRegion></affiliation></author><author><name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name><affiliation wicri:level="4"><nlm:aff id="aff8">Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp, Belgium</nlm:aff><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Medical Microbiology, Vaccine and Infectious Diseases Institute, University of Antwerp, Antwerp</wicri:regionArea><orgName type="university">Université d'Anvers</orgName><placeName><settlement type="city">Anvers</settlement><region>Région flamande</region><region type="district" nuts="2">Province d'Anvers</region></placeName></affiliation></author><author><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><affiliation wicri:level="1"><nlm:aff id="aff4">National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>National Institute for Public Health and the Environment (RIVM), Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author><author><name sortKey="Koopmans, Marion Pg" sort="Koopmans, Marion Pg" uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name><affiliation wicri:level="3"><nlm:aff id="aff3">Department of Viroscience, Erasmus MC, Rotterdam, the Netherlands</nlm:aff><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Department of Viroscience, Erasmus MC, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><affiliation wicri:level="3"><nlm:aff id="aff1">Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><series><title level="j">Eurosurveillance</title><idno type="ISSN">1025-496X</idno><idno type="eISSN">1560-7917</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Clinical Laboratory Techniques (methods)</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Disease Outbreaks</term><term>Humans</term><term>RNA, Viral (analysis)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Flambées de maladies</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Sensibilité et spécificité</term><term>Techniques de laboratoire clinique ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Clinical Laboratory Techniques</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Outbreaks</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coronavirus</term><term>Flambées de maladies</term><term>Humains</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de laboratoire clinique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur.</p></sec><sec><title>Aim</title><p>We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available.</p></sec><sec><title>Methods</title><p>Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.</p></sec><sec><title>Results</title><p>The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project.</p></sec><sec><title>Conclusion</title><p>The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Stohr, K" uniqKey="Stohr K">K Stöhr</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Abbott, A" uniqKey="Abbott A">A 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Pas</name></author><author><name sortKey="Patel, P" uniqKey="Patel P">P Patel</name></author><author><name sortKey="Reusken, C" uniqKey="Reusken C">C Reusken</name></author><author><name sortKey="Domingo, C" uniqKey="Domingo C">C Domingo</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gobat, N" uniqKey="Gobat N">N Gobat</name></author><author><name sortKey="Amuasi, J" uniqKey="Amuasi J">J Amuasi</name></author><author><name sortKey="Yazdanpanah, Y" uniqKey="Yazdanpanah Y">Y Yazdanpanah</name></author><author><name sortKey="Sigfid, L" uniqKey="Sigfid L">L Sigfid</name></author><author><name sortKey="Davies, H" uniqKey="Davies H">H Davies</name></author><author><name sortKey="Byrne, Jp" uniqKey="Byrne J">JP Byrne</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>Belgique</li><li>France</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>République populaire de Chine</li></country><region><li>Angleterre</li><li>Berlin</li><li>Grand Londres</li><li>Hollande-Méridionale</li><li>Provence-Alpes-Côte d'Azur</li><li>Province d'Anvers</li><li>Région flamande</li></region><settlement><li>Anvers</li><li>Berlin</li><li>Londres</li><li>Marseille</li><li>Rotterdam</li></settlement><orgName><li>Université d'Aix-Marseille</li><li>Université d'Anvers</li></orgName></list><tree><country name="Allemagne"><region name="Berlin"><name sortKey="Corman, Victor M" sort="Corman, Victor M" uniqKey="Corman V" first="Victor M" last="Corman">Victor M. Corman</name></region><name sortKey="Bleicker, Tobias" sort="Bleicker, Tobias" uniqKey="Bleicker T" first="Tobias" last="Bleicker">Tobias Bleicker</name><name sortKey="Brunink, Sebastian" sort="Brunink, Sebastian" uniqKey="Brunink S" first="Sebastian" last="Brünink">Sebastian Brünink</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Kaiser, Marco" sort="Kaiser, Marco" uniqKey="Kaiser M" first="Marco" last="Kaiser">Marco Kaiser</name><name sortKey="Landt, Olfert" sort="Landt, Olfert" uniqKey="Landt O" first="Olfert" last="Landt">Olfert Landt</name><name sortKey="Schmidt, Marie Luisa" sort="Schmidt, Marie Luisa" uniqKey="Schmidt M" first="Marie Luisa" last="Schmidt">Marie Luisa Schmidt</name><name sortKey="Schneider, Julia" sort="Schneider, Julia" uniqKey="Schneider J" first="Julia" last="Schneider">Julia Schneider</name></country><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="Molenkamp, Richard" sort="Molenkamp, Richard" uniqKey="Molenkamp R" first="Richard" last="Molenkamp">Richard Molenkamp</name></region><name sortKey="Goderski, Gabriel" sort="Goderski, Gabriel" uniqKey="Goderski G" first="Gabriel" last="Goderski">Gabriel Goderski</name><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name><name sortKey="Koopmans, Marion Pg" sort="Koopmans, Marion Pg" uniqKey="Koopmans M" first="Marion Pg" last="Koopmans">Marion Pg Koopmans</name><name sortKey="Meijer, Adam" sort="Meijer, Adam" uniqKey="Meijer A" first="Adam" last="Meijer">Adam Meijer</name><name sortKey="Mulders, Daphne Gjc" sort="Mulders, Daphne Gjc" uniqKey="Mulders D" first="Daphne Gjc" last="Mulders">Daphne Gjc Mulders</name><name sortKey="Reusken, Chantal" sort="Reusken, Chantal" uniqKey="Reusken C" first="Chantal" last="Reusken">Chantal Reusken</name><name sortKey="Van Den Brink, Sharon" sort="Van Den Brink, Sharon" uniqKey="Van Den Brink S" first="Sharon" last="Van Den Brink">Sharon Van Den Brink</name><name sortKey="Van Der Veer, Bas" sort="Van Der Veer, Bas" uniqKey="Van Der Veer B" first="Bas" last="Van Der Veer">Bas Van Der Veer</name><name sortKey="Wijsman, Lisa" sort="Wijsman, Lisa" uniqKey="Wijsman L" first="Lisa" last="Wijsman">Lisa Wijsman</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Chu, Daniel Kw" sort="Chu, Daniel Kw" uniqKey="Chu D" first="Daniel Kw" last="Chu">Daniel Kw Chu</name></noRegion><name sortKey="Peiris, Malik" sort="Peiris, Malik" uniqKey="Peiris M" first="Malik" last="Peiris">Malik Peiris</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Romette, Jean Louis" sort="Romette, Jean Louis" uniqKey="Romette J" first="Jean-Louis" last="Romette">Jean-Louis Romette</name></region></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Ellis, Joanna" sort="Ellis, Joanna" uniqKey="Ellis J" first="Joanna" last="Ellis">Joanna Ellis</name></region><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name></country><country name="Belgique"><region name="Région flamande"><name sortKey="Goossens, Herman" sort="Goossens, Herman" uniqKey="Goossens H" first="Herman" last="Goossens">Herman Goossens</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|area= SrasV1
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|texte= Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
}}
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